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Fig. 5 | Virology Journal

Fig. 5

From: Transcriptome analysis of Nicotiana benthamiana infected by Tobacco curly shoot virus

Fig. 5

Comparison of relative expression levels determined by RNA-seq and qPCR on 12 genes selected. All qPCR reactions were used three biological replica samples, repeated three times, and the data are presented as the mean ± SD. Relative expression levels are calculated from Ct values according to the 2–ΔΔCt method. Actin was the reference gene for these qPCR experiments. Asterisks indicate statistically significant differences compared with CK, “*” indicate significant difference (0.01 ≤ p < 0.05), “**” indicate extremely significant difference (P < 0.01)

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