Fig. 5

2B11 and 2G8 neutralization against PEDV interference of virus attachment to cells. a PEDV attachment to cells. For the 37 °C attachment, PEDV was first incubated with antibody for 1 h and then added to cells for 1 h infection incubation at 37 °C. For the 4 °C attachment, PEDV was initially incubated with Vero cells for 1 h at 4 °C, and antibody was then added to the PEDV-cell culture and the temperature was increased to 37 °C for 1 h infection incubation. The cell-associated PEDV was quantified by viral RNA RT-qPCR. 9G11 was set as an isotype control. b Comparison of epitopes between 2G8 and 2B11 nAbs using a competition ELISA test. 2G8 was used as a competitor, and 9G11 was used as a negative control. The results are presented as the mean ± SEM (n = 3). Differences were considered significant if the P value was < 0.05. P values are indicated as follows: *P < 0.05; ***P < 0.001