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Fig. 3 | Virology Journal

Fig. 3

From: Ubiquitin-conjugating enzyme UBE2J1 negatively modulates interferon pathway and promotes RNA virus infection

Fig. 3

UBE2J1 promotes ZIKV, H1N1 and SeV infection. a-f viral mRNA copies and viral titers of ZIKV (a and b), H1N1 (c and d) and SeV in (e and f) in UBE2J1 silenced (a, c, and e) or overexpressed (b, d, and f) HEK293T cells compared with controls. HEK293T cells were transfected with UBE2J1 shRNA or UBE2J1 overexpression plasmids for 24 h, and then infected with ZIKV (MOI of 1 for 24 h), H1N1 (MOI of 1 for 24 h) or SeV (MOI of 1 for 16 h), respectively. The intracellular viral loads, in terms of transcript levels of the specific viral genes (Additional file 1: Table S1), were quantified through qRT-PCR and normalized to β-actin gene. The viral titers were determined by TCID50 assay (for ZIKV) or hemagglutination assay (for H1N1 and SeV, HA units (HAU) /ml). Results were expressed as the mean + SD. * p < 0.05 and ** p < 0.01 (t-test). Representative results from at least 3 independent experiments

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