Fig. 2
UBE2J1 promotes DENV infection. a RNAi efficiency of UBE2J1 shRNA in HEK293T cells. b Silencing UBE2J1 by RNAi and infecting cell with DENV for 24 h or 48 h (MOI of 1) showed no cytotoxic effect to HEK293T cells (Cell Viability assay, Promega). c DENV viral loads in UBE2J1 shRNA (or scramble shRNA, N.C.) treated HEK293T cells at 12 h, 24 h, and 48 h post DENV infection (MOI of 1). The viral burdens were analyzed by measuring the virus E gene expression using qRT-PCR and western blot, and normalized to human β-actin or GAPDH, respectively. d qRT-PCR and western blot analysis of DENV E gene expression in HEK293T cells with or without UBE2J1 overexpression at 12 h, 24 h, and 48 h post DENV infection. e and f Viral titers in supernatants of DENV infected HEK293T cells determined by TCID50 assay on Vero cells. g-h qRT-PCR analysis of viral mRNAs and TCID50 assay of viral titers in K562 cells transfected with UBE2J1 shRNA or UBE2J1 overexpression plasmids post DENV infection. Results were expressed as the mean + SD. * p < 0.05 and ** p < 0.01 (t-test). Representative results from at least 3 independent experiments