Sample | Pretreatment conditions | Probe concentration | AMP5 incubation time | Washes after probe and AMPs incubation | Substrate incubation | Observations | ||
---|---|---|---|---|---|---|---|---|
Target Retrieval | Hydrogen Peroxide | Protease Plus | ||||||
N. rustica (stem) | 15 min | 10 min | 30 min | not diluted | 30 min | 2 W, 2 min each | 10 min | Strong background |
5 min | 10 min | 10 min | not diluted | 15 min | 3 W, 5 min each | 2 min | Background reduced but low signal | |
15 min | 10 min | 15 min | not diluted | 15 min | 3 W, 5 min each | 2 min | Significant background | |
15 min | 10 min | 15 min | not diluted | 10 min | 3 W, 5 min each | 2 min | Significant background | |
15 min | 10 min | 15 min | 1:10 in diluent buffer | 10 min | 3 W, 5 min each | 2 min | Minimal background | |
15 min | 10 min | 15 min | 1:40 in diluent buffer | 10 min | 3 W, 5 min each | 2 min | Minimal background | |
Cassava (stem) | 15 min | 10 min | 15 min | not diluted | 10 min | 3 W, 5 min each | 8 min | Absence of background |
15 min | 10 min | 15 min | not diluted | 15 min | 3 W, 5 min each | 8 min | Absence of background | |
Cassava (leaf, petiole) | 15 min | 10 min | 15 min | not diluted | 10 min | 3 W, 5 min each | 8 min | Absence of background |
15 min | 10 min | 15 min | not diluted | 15 min | 3 W, 5 min each | 8 min | Absence of background |