Survey for major viruses in commercial Vitis vinifera wine grapes in Ontario

Xiao, Huogen; Shabanian, Mehdi; Moore, Clayton; Li, Caihong; Meng, Baozhong

doi:10.1186/s12985-018-1036-1

Virology Journal

Table 2 Primers used for RT-PCR (or PCR) detection of target grapevine viruses

From: Survey for major viruses in commercial Vitis vinifera wine grapes in Ontario

Groups	Viruses	Primers	Sequences (5′-3′)	Product (bp)	Target gene
A	GRBV	GRBaV685F	GAGGGTTGTTTGAAGATAAAG	719	CP
	GRBV	GRBaV1403R	CCATAATAAACAGCGTGGTC	719	CP
	GLRaV-3	LR3-CP107F	TCTTAAARTAYGTTAAGGACGG	301	CP
	GLRaV-3	LR3-CP407R	GGCTCGTTAATAACTTTCGG	301	CP
	GLRaV-1	LR1-502F	TTGAGRGCTCTBATAAAYGAAC	379	HSP70h
	GLRaV-1	LR1-880R	CGTTMARTTCGYCKACSGACA	379	HSP70h
	GLRaV-2	LR2-14568F	RCDATGGAGYTRATGTCYGA	525	CP
	GLRaV-2	LR2-15092R	AGCGTACATRCTYGCRAACA	525	CP
B	GVA	GVA6538F	TCTTCGGGTACATCGCCTTG	325	CP
	GVA	GVA6862R	TCRAACATAACCTGTGGYTC	325	CP
	GRSPaV	RSP35	AGRYTTAGRGTRGCTAARGC	476	Rep
	GRSPaV	RSP36	CACATRTCATCVCCYGCAAA	476	Rep
	GVB	GVB6448F	ATGGAAAATATATCCCKGATGG	603	CP
	GVB	GVB7050R	GTTAACCACCTATATYTCRACAG	603	CP
C	ToRSV	ToRSV-1	CAGGAAGGTACAGACGCC	340	CP
	ToRSV	ToRSV-2	ACGTCCACGTAACTTCTGG	340	CP
	GLRaV-4	LR4-13269F	GGACAATTTAGGTAATGTWGTRGCTAC	490	P23 (ORF6)
	GLRaV-4	LR4-13758R	TATCCTCAGWGAGGAARCGG	490	P23 (ORF6)
	GFkV	GFkV5209F	GTCCTCGGCCCAGTGAAAAAG	348	Rep
	GFkV	GFkV-5556R	CAGGTTGTAGTCGGTGTTGTC	348	Rep
D	GFLV	GFLV3135F	TTGAGATTGGWTCYCGTTTC	558	CP
	GFLV	GFLV3692R	CTGTCGCCACTAAAAGCATG	558	CP
	GAMaV	GAMaV6083F	CTCGCGCTCCTCGCATTGTT	467	CP
	GAMaV	GAMaV6549R	CGTGACGAGGTTGGTCCCA	467	CP
	GSyV-1	GSyV5725F	ATGATGCAACCGACCCTTCC	671	CP
	GSyV-1	GSYV6395R	TGGAGGCTTTATTCAGAGAG	671	CP
	ArMV	ArMV2291F	CRGGTATTACGTGGGTTATGAG	292	CP
	ArMV	ArMV2582R	GCTGCCTCAAACTCAGCATA	292	CP
E	GPGV	GPGV6586F	GAYATGTCGATTCGTCAGGAG	436	CP
	GPGV	GPGV7021R	CGACTTCTGGTGCCTTATCAC	436	CP
	GLRaV-7	LR7-12163F	CTAGTGAATTACACCGAGAAGTC	550	CP
	GLRaV-7	LR7-12712R	GTGACTTGGCACGCATGTATC	550	CP
	GRVFV	GRVFV5646F	GTYGAARTCTCTCTCTTCTCCCA	389	CP
	GRVFV	GRVFV6034R	ATTATGAGAGCAACCCACTGGAAG	389	CP
	Internal reference gene	UBI-F	CCGCACTCTTGCTGATTACA	146	Ubiquitin-ribosomal protein L40–2
	Internal reference gene	UBI-R	GTGCATAACATTTGCGGCAG	146	Ubiquitin-ribosomal protein L40–2

The full names of viruses that are included in this survey are provided in the list of abbreviations. CP capsid protein, HSP70h heat shock protein 70 homologue, Rep replicase protein

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ISSN: 1743-422X

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