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Fig. 2 | Virology Journal

Fig. 2

From: Human β-defensin 2 plays a regulatory role in innate antiviral immunity and is capable of potentiating the induction of antigen-specific immunity

Fig. 2

Quantitative analysis of type I/II/III interferon and chemokine profiles in HBD 2-conjugated S RBD-treated THP-1 cells. Cells were stimulated with 1 μg/mL recombinant S RBD protein with (S RBD-HBD 2) or without (S RBD) HBD 2 conjugation, and protein levels of (a) IFN-β, (b) IFN-γ, (c) IFN-λ, (d) CXCL-1, (e) CXCL-10, (f) MCP-1, (g) MIP-1α, (h) MIP-1β, and (i) RANTES were measured from culture supernatant by cytometric bead assay, as described in Materials and Methods, 6 h and 24 h after treatment. The reactions were performed in duplicate for every target protein in each experiment. The concentrations of each protein in the PBS, S RBD, and S RBD-HBD 2 supernatants were determined by comparison with the standard curve for target proteins. Means ± SDs are shown for each time point. * p < 0.05 and *** p < 0.001 indicate significant differences between groups

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Virology Journal

ISSN: 1743-422X