Table 1 Nucleotide sequences of primers used for screening and sequencing in this study
From: Detection and genetic characterization of feline bocavirus in Northeast China
Primer name | Nucleotide sequence (5′-3′) | Product (bp) | Application |
|---|---|---|---|
FBoV1F | TCTACAAGTGGGACATTGGA | 133 | Screening for FBoV-1 [11] |
FBoV1R | GAGCTTGATTGCATTCACGA | ||
FBoV2F | TCGTTCGTCTTGGAACATAGC | 331 | Screening for FBoV-2 [17] |
FBoV2R | CAGAGCGTGGATCTGTCTGA | ||
FBD1L1 | TGACTCGTCTGTGGCGGGCT | 546 | Screening for FBoV-3 [27] |
FBD1R1 | TCGTTCGTGAGACGCTGCCA | ||
FBOV1-NS1F | TTTGGGGCTGAAGTCTGCTATGC | 705 | Sequencing for partial NS1 gene of FBoV-1 |
FBOV1-NS1R | ATGCGGCTGAGATGTACCTTGACC | ||
FBOV2-NS1F | TTCGCGGATCCAGCATACACCTAC | 963 | Sequencing for partial NS1 gene of FBoV-2 |
FBOV2-NS1R | CTCACAACGGCGCAAGCAGTCTA | ||
FBoV1-55Fa | CCGGCGCGATGACGTGTCAG | 523 | Sequencing for the complete genome of FBoV-1 |
FBoV1-577R | GCGCCGCATAGCAGACTTCAG | ||
FBoV1-550F | TTTGGGGCTGAAGTCTGCTATGCG | 1695 | |
FBoV1-2245R | CGATGTCGCCTCGATCGTGYAGTTC | ||
FBoV1-2177F | ATGAGACCGGAACGTGTATGAGCT | 1407 | |
FBoV1-3583R | GGCGCCCCCTCTTGTCCAT | ||
FBoV1-3553F | AGACGGCTGGGGATGGACAAGA | 1411 | |
FBoV1-4963R | GGYAGTGTGCCATCAGCCGAATC | ||
FBoV1-4734F | ATGGCTAGATGGGGTTCAGTG | 635 | |
FBoV1-5368R | GTAGCAGTGTGGAGGGTGTTGTAT | ||
FBoV2-66Fb | GATGACGTGTCAGTGTGGGTGTTG | 1278 | Sequencing for the complete genome of FBoV-2 |
FBoV2-1343R | ACGTTAGCCACAAATTATCCTCACA | ||
FBoV2-1306F | CTGCTTGCGCCGTTGTGAGGATAAT | 1102 | |
FBoV2-2407R | GTCTGGAGCACTGCCGCCGTCTG | ||
FBoV2-2384F | ACAGACGGCGGCAGTGCTCCAGAC | 1315 | |
FBoV2-3698R | CCATGCCCGCCACCCCCAGAG | ||
FBoV2-3653F | GTGGGGGTGGCAGCGGTGTTGG | 1014 | |
FBoV2-4666R | CGCCTCCCCTGTCTGGTATGTTGG | ||
FBoV2-4219F | CTAAACGCAGATAATGTGGAAAAA | 1176 | |
FBoV2-5394R | CAGTGTGGGTGATGTTGAGAAG |