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No. of animals
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25 males/25 females
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|
Groups
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low-dose, mid-dose, high-dose, Ad5-null, and vehicle control group
|
|
Subcutaneous injection
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Weekly × 7
|
|
Day killed
|
Days 46 and 71
|
|
Clinical signs
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Twice daily
|
|
Body weight and temperature
|
Twice before injection, weekly after dosing
|
|
ECG
|
Pre-injection, day 44, and day 67
|
|
Clinical pathology (hematology, serum chemistry)
|
Pre-injection, day 21, day 44, and day 67
|
|
Serum IFN-γ
|
Pre-injection, day 7, day 35, day 45, and day 68
|
|
Lymphocyte subsets
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Pre-injection, day 3, day 10, day 45, and day 68
|
|
Serum anti-Ad antibody, neutralizing antibody
|
Pre-injection, day 7, day 21, day 45, and day 68
|
|
Systemic autopsy, organ weight, histopathology
|
Day 46 and day 71
|
|
Hepatic IFN-γ, TNF-α, and IL-2
|
