Table 3 Primer information for qRT-PCR amplification
From: Generation and characterization of UL41 null pseudorabies virus variant in vitro and in vivo
Gene | Primer sequence (5′ to 3′) |
|---|---|
28S rRNA | F: GGGCCGAAACGATCTCAACC |
R: GCCGGGCTTCTTACCCATT | |
GAPDH | F: ATCCTGGGCTACACTGAGGA |
R: TGTCGTACCAGGAAATGAGCT | |
β-actin | F: CTCTCTTCCAACCTTCCTTCC |
R: CAGACTCGTCATACTCCTGCTT | |
UL41 | F: TGAAGAACGAGACGCGGG |
R: TGTGCTTCCAAAACAGGCCC | |
IE180 | F: CATCGTGCTGGACACCATCGAG |
R: ACGTAGACGTGGTAGTCCCCCA |