Fig. 7
From: Generation and characterization of UL41 null pseudorabies virus variant in vitro and in vivo
The functional analysis of host shutoff activity of UL41. a Multiple amino acid sequence alignment of the UL41 protein of different PRV strains. b Functional assay for vhs activity of the UL41 protein of different PRV strains. The HEK293T cells were co-transfected with 200 ng pGL3-control plasmid and 2 μg of plasmids expressing either empty vector (pCMV-3 × Flag), flag-tagged JS-2012 UL41, or flag-tagged SC UL41. After 36 h, the cells were harvested and luciferase readings were determined (Left). Data are representative of three independent experiments. Flag-tagged protein, β-actin and β-tubulin were detected by Western blot (Middle), and ratio of the expression of β-actin/β-tubulin was calculated by ImageJ software (Right). c The expression changes of β-actin (Left) and GAPDH (Right) at the 3 h post infection of 10 MOI of each virus infection or mock infection. All statistical analyses were performed using GraphPad Prism software with Student’s t-test, *P < 0.05, ns was refered as no significance