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Fig. 1 | Virology Journal

Fig. 1

From: Inhibition of enterovirus 71 replication and viral 3C protease by quercetin

Fig. 1

Quercetin inhibited EV71 proliferation. a The molecular structure of quercetin. b Reduction of EV71-GFP infection assay. RD cells were infected by EV71-GFP virus (100 TCID50), with or without treatments with various concentrations of quercetin. At 48 h pi, GFP expression was observed under a fluorescence microscope. c Antiviral activity was indicated by the reduction of the number of GFP-positive cells. d Antiviral activity of quercetin against EV71 in RD and Vero cells. Cells were infected with 100 TCID50 of EV71 mixed with serial dilutions of quercetin for 1.5 h, the inoculum was aspirated and cells were incubated with DMEM/quercetin for 48 h pi, the viability of the cells was determined with an MTT assay. VC, virus control. e The cytotoxicity of quercetin in RD and Vero cells. Cells were treated with serial concentrations of quercetin, the cell viability was determined by MTT assay after 48 h. f Morphology image of RD cells treated with quercetin (magnification, 20×). g The inhibitory effect of quercetin on EV71-induced apoptosis. RD cells were mock-infected or infected with EV71 (100 TCID50) in the presence or absence of quercetin (50 μM). The cells were stained with annexin-V-FITC/PI at 36–48 h pi., and cell apotosis and death was determined via a flow cytometry. The experiments were performed three times and the representative results were shown

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