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Table 1 Diagnostic parameter of Strep-rσ-1-His indirect ELISA

From: Detection of mammalian orthoreovirus type-3 (Reo-3) infections in mice based on serotype-specific hemagglutination protein sigma-1

Diagnostic parameter

Value

Repeatability

PC: 5.4% CoV (n = 36)

NC: 6.0% CoV (n = 36)

Intermediate Precision

PC: 11.2% CoV (n = 5)

NC: 15.8% CoV (n = 5)

Long-term Stability

> 70 d (4 °C); < 10 d (37 °C)

LODref (D-SN; D-SP)

0.31 (100%, n = 30; 98.2%, n = 110)

ROCref (D-SN; D-SP)

0.36 (100%, n = 30; 99.1%, n = 110)

Selectivity based on ROCref

98.8% (n = 80)

  1. Raw data of repeatability, intermediate precision, and long-term stability are listed in the supplement (Additional file 5). Ref Calculation based on sera of experimentally infected and control SPF mice, PC positive control, NC negative control, CoV coefficient of variation
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Virology Journal

ISSN: 1743-422X