Prevalence and sequence analysis of equid herpesviruses from the respiratory tract of Polish horses

Table 2 Primers and probes used for detection of equid herpesvirus 2 (EHV-2) and EHV-5 among horses in Poland

Assay	Virus	Region	Primers / probes (5′ to 3′)	Size (bp)	Reference
Real-time PCR	EHV-2	gB	Forward: GTGGCCAGCGGGGTGTTC	78	[42]
			Reverse: CCCCCAAAGGGATTYTTGAA
			Probe: FAM-CCCTCTTTGGGAGCATAGTCTCGGGG-TAMRA
	EHV-5		Forward: AACCCGCCGTGCATCA	66
			Reverse: AGGCGCCACACACCCTAA
			Probe: FAM-ACAACACCACCAACCCCTTTCTGCTG-TAMRA
Conventional PCR	EHV-2		Forward: GATGGTCTCACCTCTAGCAT	1111	[12]
			Reverse: CTGGTGTAACACAGGTCTTC
	EHV-5		Forward: CCAACACAGAAGACAAGGAG	1339
			Reverse: CACGGTGATACAGTCAGAGA

Fluorescent probes were dually labelled with carboxyfluorescein (FAM) and tetramethylrhodamine (TAMRA)

ISSN: 1743-422X