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Table 2 Precision and accuracy for the measurements of A, C, G, U, and m6A from three validation runs (2 μL of aliquot was injected in each run)

From: N6-methyl-adenosine level in Nicotiana tabacum is associated with tobacco mosaic virus

Analyte Concentration (μM) Intra-day Inter-day
Mean recovery± SD (%) RSD (%) Mean recovery± SD (%) RSD (%)
A 0.100 87.7 ± 1.1 1.2 89.3 ± 0.9 1.0
1.000 86.9 ± 4.2 4.9 97.4 ± 6.4 6.5
2.500 94.5 ± 0.7 0.8 90.6 ± 6.9 7.6
G 0.250 87.2 ± 3.5 4.0 94.8 ± 7.6 8.0
2.500 89.9 ± 0.4 0.5 99.6 ± 2.6 2.7
12.500 102.5 ± 1.9 1.9 106.3 ± 4.4 4.1
U 5.000 96.6 ± 4.3 4.3 100.3 ± 2.1 2.1
25.000 87.6 ± 9.5 9.5 100.3 ± 4.3 4.3
250.000 96.9 ± 6.9 6.9 96.5 ± 2.0 2.0
C 0.500 102.5 ± 0.4 0.3 99.2 ± 4.6 4.6
2.500 101.5 ± 8.3 8.2 98.7 ± 7.3 7.4
25.000 114.8 ± 5.7 4.9 90.0 ± 2.8 3.1
M6A 0.005 93.7 ± 2.7 2.9 91.2 ± 4.7 5.2
0.025 90.9 ± 3.2 3.5 91.4 ± 8.2 9.0
0.125 91.9 ± 2.7 2.9 95.1 ± 4.9 5.2