Table 2 Precision and accuracy for the measurements of A, C, G, U, and m6A from three validation runs (2 μL of aliquot was injected in each run)
From: N6-methyl-adenosine level in Nicotiana tabacum is associated with tobacco mosaic virus
Analyte | Concentration (μM) | Intra-day | Inter-day | ||
|---|---|---|---|---|---|
Mean recovery± SD (%) | RSD (%) | Mean recovery± SD (%) | RSD (%) | ||
A | 0.100 | 87.7 ± 1.1 | 1.2 | 89.3 ± 0.9 | 1.0 |
1.000 | 86.9 ± 4.2 | 4.9 | 97.4 ± 6.4 | 6.5 | |
2.500 | 94.5 ± 0.7 | 0.8 | 90.6 ± 6.9 | 7.6 | |
G | 0.250 | 87.2 ± 3.5 | 4.0 | 94.8 ± 7.6 | 8.0 |
2.500 | 89.9 ± 0.4 | 0.5 | 99.6 ± 2.6 | 2.7 | |
12.500 | 102.5 ± 1.9 | 1.9 | 106.3 ± 4.4 | 4.1 | |
U | 5.000 | 96.6 ± 4.3 | 4.3 | 100.3 ± 2.1 | 2.1 |
25.000 | 87.6 ± 9.5 | 9.5 | 100.3 ± 4.3 | 4.3 | |
250.000 | 96.9 ± 6.9 | 6.9 | 96.5 ± 2.0 | 2.0 | |
C | 0.500 | 102.5 ± 0.4 | 0.3 | 99.2 ± 4.6 | 4.6 |
2.500 | 101.5 ± 8.3 | 8.2 | 98.7 ± 7.3 | 7.4 | |
25.000 | 114.8 ± 5.7 | 4.9 | 90.0 ± 2.8 | 3.1 | |
M6A | 0.005 | 93.7 ± 2.7 | 2.9 | 91.2 ± 4.7 | 5.2 |
0.025 | 90.9 ± 3.2 | 3.5 | 91.4 ± 8.2 | 9.0 | |
0.125 | 91.9 ± 2.7 | 2.9 | 95.1 ± 4.9 | 5.2 | |