Table 2 Oligonucleotides used for real-time RT-PCR
OLIGONUCLEOTIDE | SEQUENCE (5’–3’) |
|---|---|
5’ M2 (INFLUENZA) | GACCRATCCTGTCACCTCTGAC |
3’ M2 (INFLUENZA) | GGGCATTYTGGACAAAKCGTCTACG |
5’ IL-1β | CTCGCCAGTGAAATGATGGCT |
3’ IL-1β | GTCGGAGATTCGTAGCTGGAT |
5’ TNF-α | ACTTTGGAGTGATCGGCC |
3’ TNF-α | GCTTGAGGGTTTGCTACAAC |
5’ IL-8 | GGTGCAGTTTTGCCAAGGAG |
3’ IL-8 | TTCCTTGGGGTCCAGACAGA |
5’ IL-10 | AGGATCAGCTGGACAACTT |
3’ IL-10 | GATGTCTGGGTCTTGGTTCTC |
5’ GAPDH (H) | GGTGGTCTCCTCTGACTTCAACA |
3’ GAPDH (H) | GTTGCTGTAGCCAAATTCGTTGT |
5’ GAPDH (D) | AGTCAAGGCTGAGAACGGGAAACT |
3’ GAPDH (D) | TCCACAACATACTCAGCACCAGCA |