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Fig. 2 | Virology Journal

Fig. 2

From: Cirsimaritin inhibits influenza A virus replication by downregulating the NF-κB signal transduction pathway

Fig. 2

Antiviral effect of CST against A/Fort Monmouth/1/1947(H1N1). a and b MDCK and THP-1 cells were infected with A/Fort Monmouth/1/1947(H1N1) at 100TCID50, and CST was added after viral infection. RBV is the positive control. At 24 h post infection, cells were frozen and melt, and virus from the supernatant was harvested. Viral titers were quantified by cytopathic effect assay. Results were expressed as log10 values of the mean viral load ± SD. c, d, e, and f MDCK and THP-1 cells were infected with 100TCID50 influenza virus. The medium was changed 2 h after virus infection, and cells were treated with DMSO or CST. The cells were harvested 18 h after infection for Western blot or 12 h after infection for qRT-PCR. **p < 0.01 significantly different from the Con group. Data are expressed as mean ± SD, and the results represent the average of three independent experiments. g MDCK cells were infected with the 100TCID50 influenza virus. After infection for 2 h, MDCK cells were treated with CST or RBV for 18 h. The cells were stained with an anti-M2 antibody and imaged by immunofluorescence microscopy. M2 staining is shown in green. Nuclei stained by Hoechst are shown in blue

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