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Fig. 6 | Virology Journal

Fig. 6

From: Inhibitor analysis revealed that clathrin-mediated endocytosis is involed in cellular entry of type III grass carp reovirus

Fig. 6

Effect of rottlerin and wortmannin on the production of progeny virus. CIK cells were treated with different inhibitors at the indicated concentrations and then infected with GCRV104 (MOI = 5) for 5 days. Uninternalized virions were removed at 1 hpi. Rt-PCR assay of virus yield in the supernatants. a and b Rottlerin (10 μM, 5 μM, 1 μM) and wortmannin (5 μM, 2 μM) inhibit GCRV104 infection. Asterisks represent a significant difference from the control (unpaired t-test, * P < 0.05 and ** P < 0.01). Western blot analysis to monitor viral replication level in CIK cells

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