Fig. 6From: Inhibitor analysis revealed that clathrin-mediated endocytosis is involed in cellular entry of type III grass carp reovirusEffect of rottlerin and wortmannin on the production of progeny virus. CIK cells were treated with different inhibitors at the indicated concentrations and then infected with GCRV104 (MOI = 5) for 5 days. Uninternalized virions were removed at 1 hpi. Rt-PCR assay of virus yield in the supernatants. a and b Rottlerin (10 μM, 5 μM, 1 μM) and wortmannin (5 μM, 2 μM) inhibit GCRV104 infection. Asterisks represent a significant difference from the control (unpaired t-test, * P < 0.05 and ** P < 0.01). Western blot analysis to monitor viral replication level in CIK cellsBack to article page