Fig. 4

Effect of inhibitors on the production of progeny virus. CIK cells were treated with different inhibitors at the indicated concentrations and then infected with GCRV104 (MOI = 5) for 5 days. Uninternalized virions were removed at 1 hpi. Rt-PCR assay of virus yield in the supernatants. a Pistop2 (5 μM and 1 μM) and CPZ (10 μM, 5 μM, and 1 mM) inhibit GCRV104 infection. b Rottlerin (5 μM and 2 μM) and wortmannin (5 μM and 2 μM) inhibit GCRV104 infection. c and d Nystatin (15 μM, 3 μM), Methyl-β-cyclodextrin (1 mM, 0.5 mM, 0.1 mM), Latrunvulin B (0.5 μM, 0.25 μM, 0.05 μM), nocodazole (10 μM, 5 μM, 1 μM), IPA-3 (10 μM, 5 μM, 1 μM), Amiloride (10 μM, 5 μM, 1 μM), and Bafilomycin A1 (2 nM, 1 nM, 0.2 nM) were used for analysis different pathways. Asterisks represent a significant difference from the control (unpaired t-test, * P < 0.05 and ** P < 0.01)