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Table 2 Mass spectrometric peptide mass fingerprinting (MS PMF) sequence data for protein spot 1

From: HIV-1 tat expression and sulphamethoxazole hydroxylamine mediated oxidative stress alter the disulfide proteome in Jurkat T cells

Peptide Information

Calc.

Mass

Observ

Mass

± da

± ppm

Start

Seq.

End

Seq.

Sequence

819.4207

819.4188

−0.0019

−2

152

158

SVDETLR

894.4315

894.4318

0.0003

0

121

128

ADEGISFR

980.5311

980.5272

−0.0039

−4

8

16

IGHPAPNFK

1107.6045

1107.5933

−0.0112

−10

111

120

TIAQDYGVLK

1196.631

1196.63

−0.001

−1

159

168

LVQAFQFTDK

1211.6742

1211.6702

−0.004

−3

141

151

QITVNDLPVGR

1359.7994

1359.7931

−0.0063

−5

129

140

GLFIIDDKGILR

1524.7917

1524.7761

−0.0156

−10

2

16

SSGNAKIGHPAPNFK

  1. Data was obtained from MASCOT®, a search engine that compared the PMF of tryptic digests of protein spot 1 against an NCBI database of known protein masses to identify the disulphide bonded protein separated by R2D gels. The protein was determined to be peroxiredoxin 1 (Homo sapiens), a member of the peroxiredoxin family of antioxidant enzymes