Skip to main content

Advertisement

Table 2 Mass spectrometric peptide mass fingerprinting (MS PMF) sequence data for protein spot 1

From: HIV-1 tat expression and sulphamethoxazole hydroxylamine mediated oxidative stress alter the disulfide proteome in Jurkat T cells

Peptide Information
Calc. Mass Observ Mass ± da ± ppm Start Seq. End Seq. Sequence
819.4207 819.4188 −0.0019 −2 152 158 SVDETLR
894.4315 894.4318 0.0003 0 121 128 ADEGISFR
980.5311 980.5272 −0.0039 −4 8 16 IGHPAPNFK
1107.6045 1107.5933 −0.0112 −10 111 120 TIAQDYGVLK
1196.631 1196.63 −0.001 −1 159 168 LVQAFQFTDK
1211.6742 1211.6702 −0.004 −3 141 151 QITVNDLPVGR
1359.7994 1359.7931 −0.0063 −5 129 140 GLFIIDDKGILR
1524.7917 1524.7761 −0.0156 −10 2 16 SSGNAKIGHPAPNFK
  1. Data was obtained from MASCOT®, a search engine that compared the PMF of tryptic digests of protein spot 1 against an NCBI database of known protein masses to identify the disulphide bonded protein separated by R2D gels. The protein was determined to be peroxiredoxin 1 (Homo sapiens), a member of the peroxiredoxin family of antioxidant enzymes