- Black squares indicate the presence of the corresponding protein spot in that cell line and red squares represent the presence of the corresponding protein spot in that cell line only after treatment with 200 μM SMX-HA. J-E6.1 + Dox: Jurkat E6.1 cells incubated with 1000 ng/ml doxycycline (Dox). Jurkat HIV: Jurkat E6.1 cells infected with HIV. *Concentrations of Dox selected to induce a level of TatGFP mRNA equivalent to that seen in HIV-infected Jurkat T cells. For the Tat101GFP and Tat72GFP cell lines 200 ng/ml Dox was used, whereas 400 ng/ml Dox was used for all other cell lines