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Fig. 2 | Virology Journal

Fig. 2

From: HIV-1 tat expression and sulphamethoxazole hydroxylamine mediated oxidative stress alter the disulfide proteome in Jurkat T cells

Fig. 2

R2D SDS-PAGE of thiol proteins from control cell lines. Samples from (a) Jurkat E6.1, (b) Jurkat-HIV, (c) GFP and (d) Jurkat E6.1 + 1000 ng/ml Dox. The sample from panel D was incubated with 1000 ng/ml doxycycline for 40 h. Cells from each cell line were then treated with 0.5% DMSO for 2 h, collected and protein was isolated. Protein lysate (85 μg) was loaded onto the first dimension gel and run for 3 h followed by an overnight run of the second dimension gel. On the left side of the diagonal on each gel are molecular weight protein standards that are enumerated to the left of panels a and c. The gels shown in panels a and b are duplicated and used as a reference in panels a and b of Fig. 3 and Additional file 1: Figure S1 and Additional file 2: Figure S2

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