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Table 2 The test of repeatability based on SYBR Green PCR system

From: Infection status of human parvovirus B19, cytomegalovirus and herpes simplex Virus-1/2 in women with first-trimester spontaneous abortions in Chongqing, China

a. The serially diluted B19V standards of 1 × 101–106 copies per ul to measure the intra and inter repeatability
  Plasmid copies(cp/μl) B19. Ct (‾x ± s) B19. CV (%)
Inter Repeatability 1 × 106 15.91 ± 0.07 0.46
  1 × 105 19.60 ± 0.08 0.41
  1 × 104 23.71 ± 0.05 0.21
  1 × 103 28.12 ± 0.25 0.91
  1 × 102 31.37 ± 0.20 0.64
  1 × 101 35.45 ± 0.63 1.80
Intra Repeatability 1 × 106 15.46 ± 0.66 4.80
  1 × 105 19.86 ± 0.53 3.38
  1 × 104 23.86 ± 0.23 1.04
  1 × 103 27.99 ± 0.25 0.66
  1 × 102 31.42 ± 0.17 0.41
  1 × 101 35.21 ± 0.86 2.85
b. The serially diluted CMV standards of 1 × 101–106 copies per ul to measure the intra and inter repeatability
  Plasmid copies(cp/μl) CMV. Ct (‾x ± s) CMV. CV (%)
Inter Repeatability 1 × 106 15.52 ± 0.16 1.03
  1 × 105 20.27 ± 0.5 2.50
  1 × 104 24.02 ± 0.69 2.87
  1 × 103 27.83 ± 0.15 0.52
  1 × 102 31.65 ± 0.61 1.94
  1 × 101 35.24 ± 0.23 0.64
Intra Repeatability 1 × 106 15.80 ± 0.43 2.93
  1 × 105 20.72 ± 0.71 3.10
  1 × 104 24.43 ± 0.75 2.47
  1 × 103 28.26 ± 0.60 2.18
  1 × 102 32.36 ± 1.08 3.21
  1 × 101 35.88 ± 0.79 2.23
c. The serially diluted HSV standards of 1 × 101–106 copies per ul to measure the intra and inter repeatability
  Plasmid copies(cp/μl) HSV. Ct (‾x ± s) HSV. CV (%)
Inter Repeatability 1 × 106 16.03 ± 0.16 1.01
  1 × 105 19.67 ± 0.11 0.56
  1 × 104 23.25 ± 0.03 0.11
  1 × 103 26.73 ± 0.18 0.66
  1 × 102 30.69 ± 0.52 1.70
  1 × 101 34.52 ± 1.06 3.07
Intra Repeatability 1 × 106 16.73 ± 0.68 4.32
  1 × 105 20.31 ± 0.63 3.20
  1 × 104 24.02 ± 0.74 3.26
  1 × 103 27.51 ± 0.75 2.90
  1 × 102 31.48 ± 0.85 2.54
  1 × 101 35.84 ± 1.22 3.17
  1. Ct Cycle threshold, CV Coefficient of Variation