Table 2 The test of repeatability based on SYBR Green PCR system

Plasmid copies(cp/μl)

B19. Ct (‾x ± s)

B19. CV (%)

Inter Repeatability

1 × 10⁶

15.91 ± 0.07

0.46

1 × 10⁵

19.60 ± 0.08

0.41

1 × 10⁴

23.71 ± 0.05

0.21

1 × 10³

28.12 ± 0.25

0.91

1 × 10²

31.37 ± 0.20

0.64

1 × 10¹

35.45 ± 0.63

1.80

Intra Repeatability

1 × 10⁶

15.46 ± 0.66

4.80

1 × 10⁵

19.86 ± 0.53

3.38

1 × 10⁴

23.86 ± 0.23

1.04

1 × 10³

27.99 ± 0.25

0.66

1 × 10²

31.42 ± 0.17

0.41

1 × 10¹

35.21 ± 0.86

2.85

Plasmid copies(cp/μl)

CMV. Ct (‾x ± s)

CMV. CV (%)

Inter Repeatability

1 × 10⁶

15.52 ± 0.16

1.03

1 × 10⁵

20.27 ± 0.5

2.50

1 × 10⁴

24.02 ± 0.69

2.87

1 × 10³

27.83 ± 0.15

0.52

1 × 10²

31.65 ± 0.61

1.94

1 × 10¹

35.24 ± 0.23

0.64

Intra Repeatability

1 × 10⁶

15.80 ± 0.43

2.93

1 × 10⁵

20.72 ± 0.71

3.10

1 × 10⁴

24.43 ± 0.75

2.47

1 × 10³

28.26 ± 0.60

2.18

1 × 10²

32.36 ± 1.08

3.21

1 × 10¹

35.88 ± 0.79

2.23

Plasmid copies(cp/μl)

HSV. Ct (‾x ± s)

HSV. CV (%)

Inter Repeatability

1 × 10⁶

16.03 ± 0.16

1.01

1 × 10⁵

19.67 ± 0.11

0.56

1 × 10⁴

23.25 ± 0.03

0.11

1 × 10³

26.73 ± 0.18

0.66

1 × 10²

30.69 ± 0.52

1.70

1 × 10¹

34.52 ± 1.06

3.07

Intra Repeatability

1 × 10⁶

16.73 ± 0.68

4.32

1 × 10⁵

20.31 ± 0.63

3.20

1 × 10⁴

24.02 ± 0.74

3.26

1 × 10³

27.51 ± 0.75

2.90

1 × 10²

31.48 ± 0.85

2.54

1 × 10¹

35.84 ± 1.22

3.17