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Fig. 5 | Virology Journal

Fig. 5

From: Development of leafhopper cell culture to trace the early infection process of a nucleorhabdovirus, rice yellow stunt virus, in insect vector cells

Fig. 5

Structural proteins N and P formed viroplasm-like structures in nonhost Sf9 cells. a Early and late infection stage of the recombinant protein N-His and P-Strep. b Recombinant baculoviruses expressed N-His and P-Strep aggregated in the nucleus and formed viroplasm-like inclusions at 72 hpi. Samples were immunostained with rabbit polyclonal antibodies against His and mouse polyclonal antibodies against Strep, then treated with the corresponding fluorescence-labeled secondary antibody. The nucleus was stained with DAPI. Bars, 10 μm. c Electron micrographs showing subcellular localization of fusion proteins N-His and P-Strep at 72 hpi in baculovirus-infected Sf9 cells. I, Healthy non-infected Sf9 cell as control. II, Sf9 cells were infected with baculovirus expressing N-His and immunolabeled with N-specific rabbit polyclonal antibody as the primary antibody; III, cells were infected with baculovirus expressing P-Strep and immunolabeled with P-specific rabbit polyclonal antibodies as the primary antibody. Cells in both II and III were treated with goat-anti-rabbit IgG conjugated to 15-nm-diameter gold particles as the secondary antibody. Insets are enlarged images of the boxed areas. Red arrows, 15-nm-diameter gold particles. Nu, nucleus. Bars, 100 nm

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