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Fig. 2 | Virology Journal

Fig. 2

From: Development of leafhopper cell culture to trace the early infection process of a nucleorhabdovirus, rice yellow stunt virus, in insect vector cells

Fig. 2

Electron micrographs showing subcellular localization of RYSV structural proteins N and P in RYSV-infected VCMs. a, b Samples were immunolabeled with (a) N- and (b) P-specific polyclonal antibodies as primary antibodies, then treated with goat-anti-rabbit IgG conjugated to 15-nm-diameter gold particles as secondary antibodies. a-I Healthy cell of VCMs as control; panels (a-III) and (b-II) are enlarged images of the boxed areas. c Double immunogold labeling electron micrographs showing the localization of RYSV structural proteins N and P in RYSV-infected VCMs. Samples were immunolabeled with N-specific rabbit polyclonal antibodies and P-specific mouse polyclonal antibodies as primary antibodies, then treated with goat-anti-rabbit IgG conjugated with 15-nm-diameter gold particles and goat-anti-mouse IgG conjugated with 10-nm-diameter gold particles. Insets are enlarged images of the boxed areas. Black arrows, 10-nm-diameter gold particles; red arrows, 15-nm-diameter gold particles. Nu, nucleus; Vi, virion(s); Vp, viroplasm. Bars, 100 nm

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