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Fig. 1 | Virology Journal

Fig. 1

From: Ebolavirus diagnosis made simple, comparable and faster than molecular detection methods: preparing for the future

Fig. 1

Analytical sensitivity of Ebolavirus RPA-LFS test. Sensitivity testing using both plasmid containing the Ebolavirus gene (top) and Zaire Ebolavirus RNA (bottom). a RT-PCR cycle threshold (Ct) values for a single sample, along with corresponding photograph of LFS with control bands (all samples) and test bands (positive samples) compared to copy number of serially diluted template DNA or RNA (copies/μL) and no template control. b Normalised pixel density (black values) from the assay displayed in (a). c Positive results compared to number of runs tested at that dilution. d Analytical sensitivity displayed as percentage of correct results from all runs. The test line appeared at every dilution down to 102 copies/μL, which was comparable with Real-Time PCR

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