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Table 5 Agreement between the tq-PCR and two step nested PCR

From: A triplex quantitative real-time PCR assay for differential detection of human adenovirus serotypes 2, 3 and 7

 

Triplex real-time PCR

Two step nest PCR

Agreement

 

Positive

Negative

Positive

Negative

 

Ad2

42

94

43

93

99.26%

Ad3

40

96

44

92

97.05%

Ad7

5

131

5

131

100%

  1. Footnote: of 138 HAdV positive samples were detected by these two methods, as nested PCR can’t show co-infection serotype 2, 7 and 2, 3, so only 136 clinical samples enrolled in agreement between the tq-PCR and two step nested PCR
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Virology Journal

ISSN: 1743-422X