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Table 5 Agreement between the tq-PCR and two step nested PCR

From: A triplex quantitative real-time PCR assay for differential detection of human adenovirus serotypes 2, 3 and 7

  Triplex real-time PCR Two step nest PCR Agreement
  Positive Negative Positive Negative  
Ad2 42 94 43 93 99.26%
Ad3 40 96 44 92 97.05%
Ad7 5 131 5 131 100%
  1. Footnote: of 138 HAdV positive samples were detected by these two methods, as nested PCR can’t show co-infection serotype 2, 7 and 2, 3, so only 136 clinical samples enrolled in agreement between the tq-PCR and two step nested PCR
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