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Table 3 Intra-assay of coefficient of variation (CV) of the tq-PCR

From: A triplex quantitative real-time PCR assay for differential detection of human adenovirus serotypes 2, 3 and 7

 

107copy/μl

105copy/μl

103copy/μl

 

Ad2

Ad3

Ad7

Ad2

Ad3

Ad7

Ad2

Ad3

Ad7

CT value

17.74

20.12

20.43

24.95

26.39

26.39

32.15

33.11

35.23

17.40

19.05

20.88

24.24

26.44

27.05

33.08

33.96

34.85

18.34

19.56

20.29

26.05

26.13

27.31

32.59

34.52

34.53

Mean

17.83

19.58

20.53

25.08

26.32

26.92

32.61

33.86

34.87

CV%

2.7%

2.7%

1.5%

3.6%

0.6%

1.8%

1.4%

2.1%

1.0%

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Virology Journal

ISSN: 1743-422X