| |
Primer (Probe)
|
Sequence (5′-3′)
|
GC content (%)
|
Tm (°C)
|
Reference
|
|---|
|
tq-PCR
|
F-primer
|
GGYCCYAGYTTYAARCCCTAYTC
|
39.13%
|
54.9 °C
|
[13] This study
|
|
R-primer
|
AAYTTGAGGYTCTGGYTGATCKG
|
39.13%
|
55.7 °C
|
|
Probe2
|
FAM-TGTGAGTGGGAACAAACCGAAG-MGB
|
50.00%
|
60.6 °C
|
|
Probe3
|
HEX-ACAATGCAGTAACTACCACCACAA-MGB
|
41.67%
|
59.6 °C
|
|
Probe7
|
Cy5-TTACTGCAGACAACAAGCCCAT-MGB
|
45.45%
|
59.7 °C
|
|
Nested PCR
|
AdhexF1
|
TICTTTGAC ATICGIGGIGTICTIGA
|
38.46%
|
60.3 °C
|
[13]
|
|
AdhexR1
|
CTGTCIACIGCCTGRTTC CACA
|
45.45%
|
59.8 °C
|
|
AdhexF2
|
GGYCCYAGYTTYAARCCCTAYTC
|
39.13%
|
54.9 °C
|
|
AdhexR2
|
GGTTCTGTCICCCAGAGARTCIAGCA
|
50.00%
|
63.3 °C
|
