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Table 1 The primer and probe of tq-pcr and nested pcr

From: A triplex quantitative real-time PCR assay for differential detection of human adenovirus serotypes 2, 3 and 7

  Primer (Probe) Sequence (5′-3′) GC content (%) Tm (°C) Reference
tq-PCR F-primer GGYCCYAGYTTYAARCCCTAYTC 39.13% 54.9 °C [13] This study
R-primer AAYTTGAGGYTCTGGYTGATCKG 39.13% 55.7 °C
Probe2 FAM-TGTGAGTGGGAACAAACCGAAG-MGB 50.00% 60.6 °C
Probe3 HEX-ACAATGCAGTAACTACCACCACAA-MGB 41.67% 59.6 °C
Probe7 Cy5-TTACTGCAGACAACAAGCCCAT-MGB 45.45% 59.7 °C
Nested PCR AdhexF1 TICTTTGAC ATICGIGGIGTICTIGA 38.46% 60.3 °C [13]
AdhexR1 CTGTCIACIGCCTGRTTC CACA 45.45% 59.8 °C
AdhexF2 GGYCCYAGYTTYAARCCCTAYTC 39.13% 54.9 °C
AdhexR2 GGTTCTGTCICCCAGAGARTCIAGCA 50.00% 63.3 °C
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