Fig. 3
From: Oncolytic activity of a coxsackievirus B3 strain in human endometrial cancer cell lines
CV-B3/2035A-induced lytic infection of EC cells in vitro. a EC cell monolayers were either mock-infected or infected with CV-B3/2035A at an MOI of 10, and photomicrographs were taken at 72 h post-infection (× 20 original magnification), respectively. b EC cells in 96-well plates were infected with tenfold serial dilutions of CV-B3/2035A (starting MOI = 10). Following incubation at 37 °C for 72 h, cytotoxicity was measured using CCK8 assay. The mean percentage cytotoxicity (±SD) is shown from quadruplicate wells from one representative experiment. c Growth kinetics of CV-B3/2035A in EC cells in vitro. CV-B3/2035A was inoculated in triplicate onto monolayers of EC cells in 24-well plates at an MOI of 0.1. After removal of unbound virus, the cells were incubated at 37 °C and virus yield was determined at selected time points (2, 24 and 48 h) after freezing-thawing by TCID50 assay on RD cells