Fig. 4

Effect of NS1 mutations on recombinant virus-induced cytokine expression. a The 293 T cells were infected with rSIV or NS1 mutant viruses at an MOI of 1 for 24 h. IFN-α/β and TNF-α mRNA levels in the cell lysates were quantified by real-time PCR. The fold changes in the mRNA expression levels were calculated by the comparative CT method. “MOCK” represents samples from uninfected control cells. b The 293 T infected with rSIV or NS1 mutant viruses at an MOI of 1 for 24 h were harvested for western blotting analysis of IRF3, p-IRF3 and the β-actin level. c The 293 T cells were transfected with pNFκB-luc (100 ng) and then infected with wt or NS1 mutant viruses at an MOI of 1 for 24 h prior to lysis. The constitutive activity of the promoters was measured and is presented as the relative firefly luciferase activity