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Fig. 3 | Virology Journal

Fig. 3

From: Effects of the S42 residue of the H1N1 swine influenza virus NS1 protein on interferon responses and virus replication

Fig. 3

NS1 mutations S42P alter virus replication on MDCK cells. a The growth kinetics of the recombinant viruses in MDCK cells. MDCK cells were infected with a wt or mutated virus (rSIV NS1 S42P, rSIV NS1 D92E and rSIV NS1 S42P/ D92E) at an MOI of 0.001. At 12, 24, 48, and 72 hpi, the virus titers in the supernatants were determined using a TCID50 assay. The mean values from three independent experiments are shown for each sample. b Expression kinetics of the viral proteins during infection. MDCK cells on 6-well plates were infected at an MOI 0.001 with wt or mutant viruses and the cell lysates that were collected at the indicated time points. The amounts of NS1 and actin in the lysates were examined by western blot. c Percentage of the NS1 protein reduction level. The percentage of protein reduction (%) was calculated by the formula [(NS1 expression of wt infected cells - NS1 expression of mutant viruses infected cells)/ NS1 expression of wt infected cells] × 100%; Abscissa: the time points post infection

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