Fig. 2From: Effects of the S42 residue of the H1N1 swine influenza virus NS1 protein on interferon responses and virus replicationIntracellular localization of NS1 during WT and mutant infection in MDCK cells. MDCK cells were infected with the WT and mutated virus (S42P, D92E, S42P/D92E) at an MOI of 0.001. At 6(a), 12(b), and 24(c) hpi, the cells were fixed and stained with anti-NS1 antibody and DAPI. The localizations of NS1 proteins were visualized using a Nikon Eclipse 80i fluorescence microscope. NS1: stained with FITC-labelled goat Anti-Mouse IgG (green); Nuclei: stained with DAPI (blue); Merge: merged image of double immunofluorescent staining of the same fieldBack to article page