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Fig. 1 | Virology Journal

Fig. 1

From: Effects of the S42 residue of the H1N1 swine influenza virus NS1 protein on interferon responses and virus replication

Fig. 1

Generation of NS1 mutant viruses. a Schematic representation of influenza A/Swine/Shanghai/3/2014 NS1 protein and the mutations employed in this study. b NS1 amplification of the WT and NS1 mutant viruses. The viral genomes were amplified using RT-PCR and the length of the NS1 vRNA amplicons was examined using agarose gel electrophoresis. Lane 1, 10,000 bp marker; Lane 2, WT; Lane 3, NS1 S42P; Lane 4, NS1 D92E; and Lane 5, NS1 S42P/D92E. c Western blot analyses. Whole cell lysates obtained from MDCK cells infected with mutants (rSIV NS1 S42P, rSIV NS1 D92E and rSIV NS1 S42P/ D92E) and wild-type viruses at an MOI of 0.001 for 24 h were subjected to SDS-PAGE and western blot analysis

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