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Fig. 1 | Virology Journal

Fig. 1

From: Rescue and characterization of recombinant cedar virus, a non-pathogenic Henipavirus species

Fig. 1

Schematic representation of the recombinant viral genomes. a Cloned rCedPV DNA fragments were inserted between a T7 RNA promoter and a hepatitis delta virus ribozyme in the pOLTV5 vector to yield rCedPV-wt. A MluI restriction site was created between the P and M genes to facilitate insertion of a modified turbo Green Fluorescent Protein (GFP) gene to generate rCedPV-GFP. b The 3′ terminus of the GFP gene is flanked by the P gene transcriptional stop, intergenic region (IGR), M gene transcriptional stop sequences. A non-coding thymine (T) between the M gene transcriptional stop and MluI sequence was added to preserve the rule of six necessary for replication

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