Fig. 6

In vitro interaction of wt- and mutated RBDs with model dsRNA (electrophoretic mobility shift assay). ³²P-labeled, synthetic RNA shDM03 (depicted above as MFold-predicted structure) at a final concentration of 0.1 nMol.L^− 1 was incubated with increasing concentrations (as indicated) of the dimeric wt-RBD (right panel) or mutated-RBD (left panel). RNA-protein complexes were electrophoretically separated in a 10% non-denaturing polyacrylamide gel, and were subsequently revealed using a Typhoon-Trio laser scanner