Fig. 6
From: Inhibition of HIV early replication by the p53 and its downstream gene p21
Analysis of function of p53 and its downstream gene p21 during HIV infection in hMDMs. a and b Analysis of p53, p-p53 (S15) and p21 proteins in hMDMs after HIV-1 infection by Western blot. 3.0 MOI VSV-G pseudotyped HIV-1 GFP+ virus was used to infect 0.5 × 106 hMDMs in a 24 well plate, Results from two different donors were shown. c Western blot analysis of SAMHD1, pSAMHD1 (T592) and RNR2 after siRNA knockdown of p53 and siRNA knockdown of p21 in hMDMs. d Percentages of p21 protein and p53 protein after siRNA knockdown. The non-targeting (NT) siRNA was used as negative control. e Quantification of HIV-1 Luc+ infection after siRNA knockdown of p21 and siRNA knockdown of p53 in hMDMs. f Analysis of SAMHD1 and pSAMHD1(T592) in HIV-2 infection after siRNA knockdown of p21 in hMDMs. g The percentage of p21 after siRNA knockdown in hMDMs. h Quantification of HIV-2 infection in hMDMs transfected with p21 siRNA and non-targeting siRNA. The non-targeting (NT) siRNA was used as negative control. Luciferase assay was performed at 48 h after HIV-2 Luc+ infection. The results represented a triplicate experiment. In Student’s t-test, p value < 0.05 is indicated by *