Fig. 2

Comparison of HIV-1 reverse transcription and viral cDNA degradation in infected HCT116 p53^+/+ and HCT116 p53^−/− cells. a Quantification of HIV-1 late RT in infected cycling HCT116 p53^+/+ and HCT116 p53^−/− cells and non-cycling HCT116 p53^+/+ and HCT116 p53^−/− cells 16 h post infection. b Evaluation of HIV cDNA degradation after treatment of the reverse transcriptase inhibitor EFA. The relative copy number of HIV-1 late RT product (viral cDNA) was quantified by TaqMan real time PCR. At each time point, the remaining viral cDNA (%) was calculated from the copy of cDNA per cell treated with EFA divided by the copy of cDNA per untreated cell. Time indicates the number of hours after the addition of EFA. The results represented a triplicate experiment. Comparison of HIV-1 cDNA at different stages of virus replication between non-cycling HCT116 p53^+/+ and non-HCT116 p53^−/− cells at 8 h, 16 h and 24 h post infection were performed. c Early RT. d Intermediate RT. e Late RT. f 2-LTR cycle DNA. g Integration. Both inactived virus and AZT treatment were used as negative control. Relative RT copy numbers were quantified by a TaqMan real time PCR. In Student’s t-test, p value < 0.05 is indicated by *; p value < 0.01 is indicated by **