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Fig. 1 | Virology Journal

Fig. 1

From: Inhibition of HIV early replication by the p53 and its downstream gene p21

Fig. 1

Comparison on the HIV infection between HCT116 p53+/+ and HCT116 p53−/− cells in cycling and non-cycling status. a Flow cytometry analysis of cell cycle of cycling and non-cycling HCT116 p53+/+ and HCT116 p53−/− cells. Non-cycling cells were cultured in DMEM without FBS for 24 h. Cells are stained with propidium iodide (PI) (Left). S phase cell percentages in cycling and non-cycling HCT116 p53+/+ and HCT116 p53−/− cells were also analyzed by Click-iT™ Plus EdU Flow Cytometry Assay (Right). b Cell viabilities of both cycling and non-cycling HCT116 p53+/+ and HCT116 p53−/− cells were measured by Trypan blue exclusion test (Left) and WST-1 assay (Right). c Flow cytometer analysis of infection 24 h after infection by VSV-G pseudotyped HIV-1 GFP+ virus in cycling and non-cycling HCT p53+/+ and HCT116 p53−/− cells. Uninfected cells were shown as the 1st peak, and the infected GFP+ cells were shown as the 2nd peak. d Quantification of HIV-1 GFP+ infection in cycling and non-cycling HCT116 p53+/+ and HCT116 p53−/− cells. e Quantification of infection of VSV-G pseudotyped HIV-1 Luc+ virus in cycling and non-cycling HCT116 p53+/+ and HCT116 p53−/− cells. Luciferase assay for VSV-G pseudotyped HIV-1 Luc+ virus was performed at 24 h after infection. f Quantification of infection of VSV-G pseudotyped HIV-2 Luc+ virus in cycling and non-cycling HCT116 p53+/+ and HCT116 p53−/− cells. 20 μl of transfection supernatant of pHIV-2 Luc+ and pVSV-G was used in 1 x virus infection; 100 μl of transfection supernatant was used in 5 x virus infection. Luciferase assay was performed at 48 h after HIV-2 Luc+ infection. The results represented a triplicate experiment. In Student’s t-test, p value < 0.05 is indicated by *; p value < 0.01 is indicated by **

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