Fig. 2

Sensitivity of O1 Manisa and A24 Cruzeiro and their derivatives to acidic pH Equal amounts of virus of the different passages and culture systems were incubated in buffers of different pH for 30 min, then neutralized and titrated. Titers are expressed relative to the value obtained using PBS at pH 7.5. Experiments were performed three times independently. Significance code: “***” p ≤ 0.001