Fig. 4
From: Engineering T7 bacteriophage as a potential DNA vaccine targeting delivery vector
The binding efficiency of phage particle towards eukaryotic cells. T7 phages toward target cells (Vero, MDCK, Marc-145, BHK-21). Binding efficiency of the phages were estimated as their recovery (%) = output phage / input phage. Eukaryotic cells (5.0 × 105 cells/ well) were incubated with 108 pfu phages, thoroughly washed to remove unbound phages, and then lysed. Bacteria were infected with the cell lysates to determine output phage titer by pfu assay. Data represent mean ± S.E of 3 independent tests