Substitution of amino acid residue V1213 in the helicase domain of the genotype 3 hepatitis E virus reduces virus replication

Table 1 Primers used in the generation of HEV Rluc replicons and their mutants

Primer IDs		Primer Sequence^a	Modifications^b
Replicon
	TW4675F	5’ GCTATGAGTTCCGGGACCTCAA 3’	TW6196-Rluc
	TW5168R	5’ GGTGGCATCGCCATGCAA 3’
	TWJRhRlucU	5’ TTGCATGGCGATGCCACC ATGGCTTCCAAGGTGTACGAC 3′
	TWhRlucL	5’ AGAATTCAGCGCTGCTAGCTATTACTGCTCGTTCTTCAGCACG 3’
	SWhRluc1U	5’ TGCATCGCCCATGGGATCACCATGGCTTCCAAGGTGTACGAC 3’	pSHEV3-Rluc
	SWhRluc915L	5’ CGGTTAACATATGCTAGCCTATTACTGCTCGTTCTTCAGCACG 3’
	SWHEV4623U	5’ CGCCGAAGGAGTCTCTTAAGGGT 3’
	SWHEV5208L	5’ GTCGTACACCTTGGAAGCCATGGTGATCCCATGGGCGATGCA 3’
Mutagenic
	HEV3MvaU	5’ GTGAGGTCGGCATTTCGGATGCAATTGTCAATAACTTTTTCCTTG 3’	V1213A
	HEV3MvaL	5’ CAAGGAAAAAGTTATTGACAATTGCATCCGAAATGCCGACCTCAC 3’	V1213A
	HEV3MqhU	5’ GATCAGAACCTCGGGACTCTACATGCCTTCCCGCCGTCCTGCCAG 3′	Q/H
	HEV3MqhL	5’ CTGGCAGGACGGCGGGAAGGCATGTAGAGTCCCGAGGTTCTGATC 3’	Q/H
	HEV3MspU	5’ GTCTATGGGGGCCGGGCCTCATAGCCTCACTTATG 3’	S605P
	HEV3MspL	5’ CATAAGTGAGGCTATGAGGCCCGGCCCCCATAGAC 3’	S605P
	HEV3MivU	5’ GCTTGTGCCGGCTGCACTGTTAGTCCTGGGATTG 3′	I978V
	HEV3MivL	5’ CAATCCCAGGACTAACAGTGCAGCCGGCACAAGC 3’	I978V
	HEV3MvaHU	5’ GTGAGGTCGGCATTTCGGATGCTATTGTCAATAACTTTTTCCTTG 3’	V1213A (2nd)
	TWMavU	5’ TGAGGTGGGTATCTCTGATGTTATTGTTAACAACTTCTTCC 3′	A1213V TW6196
	TWMavL	5’ GGAAGAAGTTGTTAACAATAACATCAGAGATACCCACCTCA 3′	A1213V TW6196
	HEV2MavU	5’ GCGAGGTGGGCATCTCCGATGTGATCGTTAATAACTTTTTCCTTG 3’	A1213V Sar55
	HEV2MavL	5’ CAAGGAAAAAGTTATTAACGATCACATCGGAGATGCCCACCTCGC 3’	A1213V Sar55

^aSequences of primers
^bRluc inserted into HEV genomes or amino acid mutation introduced by the primer

ISSN: 1743-422X