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Table 1 The interaction of immobilized HA-H and purified recombinant proteins

From: Quantitative investigation of the direct interaction between Hemagglutinin and fusion proteins of Peste des petits ruminant virus using surface Plasmon resonance

Sample

ka (1/Ms)

kd (1/s)

KD (kd/ka, M)

HA-F

4.91 × 104

9.37 × 10−4

1.91 × 10−8

HA-HRA

3.82 × 10

4.14 × 10−3

1.08 × 10−4

HA-HRB

1.15 × 104

2.99 × 10−3

2.60 × 10−7

HA

2.96 × 10

4.24 × 10−3

1.43 × 10−4

  1. Note: kinetic and affinity data were determined by local fitting of the binding step of the individual sensorgrams to a 1:1 L binding model. ka: association rate constant; kd: dissociation rate constant; KD: the equilibrium dissociation constant (kd/ka)