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Fig. 2 | Virology Journal

Fig. 2

From: Quantification and kinetics of viral RNA transcripts produced in Orthohantavirus infected cells

Fig. 2

Genetic organization indicating the selected nucleotides of the S, M and L-segments of Puumala Orthohantavirus used for in vitro transcription and qRT-PCR. Fig. 2a. The five colored boxes (S1, M1, M2, L1 and L2), indicate the regions of the three segments that were fused and cloned side-by-side as synthetic DNA into the plasmid pCR™II-TOPO®pUmU/Kz. Below each box, the primers and the location in the corresponding RNA transcripts are shown. Fig. 2b. The chosen regions of the different segments are color-coded in red (S-segment), black (M-segment) and blue (L-segment) according, to their origin and positions in the corresponding viral RNA of Puumala Orthohantavirus, and visulized by using SnapGene software(GSL Biotech, Chicago, IL)

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