Identification and genome analysis of tomato chlorotic spot virus and dsRNA viruses from coinfected vegetables in the Dominican Republic by high-throughput sequencing

Table 1 Primers used for amplification of virus sequences of symptomatic vegetables from Dominican Republic

Primer Name	Sequence 5ʼ - 3ʼ	Tm [°C]	Anneling Temperature [°C]
Amalga – F^a	TGG GTA TCG ACA AGC GCT AC	60.5	55
Amalga – R^a	ACA TGT CGA AGG CCT CCT TG	60.5	55
Bell – F^b	CGC TTC GAG CAT AAA AGC CC	60.5	55
Bell – R^b	TGG CTT GCG CTT TTG TGT AC	58.4	55
Pep70 – F^c	CAC CCG CAC ACA ATT AAC GG	60.5	55
Pep70 - R^c	ACA CAT CTT CGG TCC GAC AC	60.5	55
Pep126 – F^d	ACG CCC CCT ATA ACG CAA AA	58.4	55
Pep126 – R^d	AAT GTC GCA AGG GCC CAT AA	58.4	55

ISSN: 1743-422X