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Fig. 3 | Virology Journal

Fig. 3

From: Identification and genome analysis of tomato chlorotic spot virus and dsRNA viruses from coinfected vegetables in the Dominican Republic by high-throughput sequencing

Fig. 3

Tospovirus phylogeny. Trees were based on amino acid sequences of the structural proteins RNA-dependent RNA polymerase (RdRp), the nucleocapsid (N) and the glycoprotein (GnGc) and the non-structural movement (Nsm) and the silence suppressor (Nss) proteins. The species included in the phylogenetic analysis were Bean necrotic spot virus (BeNMV), Capsicum chlorosis virus (CaCV), Groundnut bud necrosis virus (GBNV), Groundnut ringspot virus (GRSV), Groundnut ringspot virus/Tomato chlorotic spot virus (reassortant) (GR/TC), Chrysanthemum stem necrosis virus (CSNV), Impatiens necrotic spot virus (INSV), Iris yellow spot virus (IYSV), Melon yellow spot virus (MYSV), Polygonum ringspot virus (PolRSV), Tomato chlorotic spot virus (TCSV), Tomato necrotic spot-associated virus (TNSaV), Tomato spotted wilt virus (TSWV), Tomato zonate spot virus (TZSV), Watermelon silver mottle virus (WSMoV) and Zucchini lethal chlorosis virus (ZLCV). The GenBank accession numbers of the sequences are shown in the trees. Nodes values with posterior probability > 50% are shown. Protein sequences were used to verify the phylogenetic relationship of the Tomato chlorotic spot virus (TCSV) isolate reported in Dominican Republic (GenBank accession numbers KX463272 [L RNA], KX463273 [M RNA], KX463274 [S RNA]). The proteins encoded at S and L RNAs clustered with the TCSV isolate already reported (GenBank accession numbers AF282982 [S RNA] and HQ700667 [L RNA]), while those encoded at M RNA are closer to the hybrid North American TCSV/GRSV (reassortant) isolate (GenBank accession number HQ644141)

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