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Fig. 6 | Virology Journal

Fig. 6

From: Development and characterization of serotype-specific monoclonal antibodies against the dengue virus-4 (DENV-4) non-structural protein (NS1)

Fig. 6

Analysis of optimal capture/detector MAb pair for development of DENV-4 serotype specific NS1 capture ELISA. The curves represent limit of detection (LOD) curve for matched pairs: a 8A6F2 and biotinylated 6D4B10, and b 6D4B10 and biotinylated 8A6F2. The optical density (OD) values at 450 nm were obtained at various concentration of DENV-4 rNS1 with an optimal concentrations for coating antibody, 5-10 μg/ml; capture antibody, 1:2000 dilution and streptavidin-tagged horseradish peroxidase (HRP-SP), 1:2000. WNV was used as the control

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