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Fig. 4 | Virology Journal

Fig. 4

From: Development and characterization of serotype-specific monoclonal antibodies against the dengue virus-4 (DENV-4) non-structural protein (NS1)

Fig. 4

Serotype-specificity of monoclonal antibodies (MAbs 3H7A9, 8A6F2, 6D4B10 and 10H10B5) against DENV-4 determined by iELISA. The bars show mean optical density (OD) at 450 nm which measures MAb reactivity to NS1s to all four DENV-4 serotypes, yellow fever virus (YFV), West Nile virus (WNV) and SUMO protein. MAbs 3H7A9, 8A6F2, and 6D4B10 represent anti-NS1 MAbs specific to DENV-4. MAb 10H10B5, which is reactive to SUMO*, represents one of the three non-specific MAbs generated from immunization of SUMO*-DENV-4 rNS1 fusion protein. The OD value for DENV-4-serotype specific MAbs against rNS1 of other flaviviruses and recombinant SUMO protein was < 0. By contrary, MAbs reactive only to SUMO* protein (10H10B5) did not show any reactivity to rNS1 to YFV and WNV

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Virology Journal

ISSN: 1743-422X