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Table 2 Qualitative results of serological investigations (seropositive/total samples analysed per assay)

From: Engineered recombinant protein products of the avian paramyxovirus type-1 nucleocapsid and phosphoprotein genes for serological diagnosis

serum panel HI assay Commercial ELISA Recombinant ELISA
NDV LS APMV-8 NP full-length Pu LS NPct APMV-8 NPct
SPF 0/51 0/51 0/51 0/51 0/51 0/51 0/51
Experimental Infection NDVa 68/83 n.d. 68/83 69/83 58/83 68/83 0/83
Experimental Infection APMV-8b 0/15 15/15 0/15 0/15 n.d. 0/15 14/15
Field sera chicken 60/150 n.d. 103/150 91/150 90/150 79/150 0/150
Field sera turkey 67/147 n.d. 113/147 100/147 57/147 88/147 0/147
  1. aExperimental NDV infection sera were obtained from chickens after immunization with a live attenuated vaccine based on NDV strain “Clone 30”; on day 21 post vaccination all chickens were challenged with thes velogenic NDV strain “Herts”
  2. bExperimental APMV-8 infection sera originated from chickens that were immunized with wildtype APMV-8 strain APMV-8/goose/Delaware/1053/76 one day after hatch and challenged with APMV-8 on day 21 postvaccination. Samples used here for serological analyzes were taken on days 21 post vaccination and 14 post challenge