serum panel
|
HI assay
|
Commercial ELISA
|
Recombinant ELISA
|
---|
NDV LS
|
APMV-8
|
NP full-length
|
Pu
|
LS NPct
|
APMV-8 NPct
|
---|
SPF
|
0/51
|
0/51
|
0/51
|
0/51
|
0/51
|
0/51
|
0/51
|
Experimental Infection NDVa
|
68/83
|
n.d.
|
68/83
|
69/83
|
58/83
|
68/83
|
0/83
|
Experimental Infection APMV-8b
|
0/15
|
15/15
|
0/15
|
0/15
|
n.d.
|
0/15
|
14/15
|
Field sera chicken
|
60/150
|
n.d.
|
103/150
|
91/150
|
90/150
|
79/150
|
0/150
|
Field sera turkey
|
67/147
|
n.d.
|
113/147
|
100/147
|
57/147
|
88/147
|
0/147
|
- aExperimental NDV infection sera were obtained from chickens after immunization with a live attenuated vaccine based on NDV strain “Clone 30”; on day 21 post vaccination all chickens were challenged with thes velogenic NDV strain “Herts”
- bExperimental APMV-8 infection sera originated from chickens that were immunized with wildtype APMV-8 strain APMV-8/goose/Delaware/1053/76 one day after hatch and challenged with APMV-8 on day 21 postvaccination. Samples used here for serological analyzes were taken on days 21 post vaccination and 14 post challenge