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Table 2 Qualitative results of serological investigations (seropositive/total samples analysed per assay)

From: Engineered recombinant protein products of the avian paramyxovirus type-1 nucleocapsid and phosphoprotein genes for serological diagnosis

serum panel

HI assay

Commercial ELISA

Recombinant ELISA

NDV LS

APMV-8

NP full-length

Pu

LS NPct

APMV-8 NPct

SPF

0/51

0/51

0/51

0/51

0/51

0/51

0/51

Experimental Infection NDVa

68/83

n.d.

68/83

69/83

58/83

68/83

0/83

Experimental Infection APMV-8b

0/15

15/15

0/15

0/15

n.d.

0/15

14/15

Field sera chicken

60/150

n.d.

103/150

91/150

90/150

79/150

0/150

Field sera turkey

67/147

n.d.

113/147

100/147

57/147

88/147

0/147

  1. aExperimental NDV infection sera were obtained from chickens after immunization with a live attenuated vaccine based on NDV strain “Clone 30”; on day 21 post vaccination all chickens were challenged with thes velogenic NDV strain “Herts”
  2. bExperimental APMV-8 infection sera originated from chickens that were immunized with wildtype APMV-8 strain APMV-8/goose/Delaware/1053/76 one day after hatch and challenged with APMV-8 on day 21 postvaccination. Samples used here for serological analyzes were taken on days 21 post vaccination and 14 post challenge