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Fig. 2 | Virology Journal

Fig. 2

From: Engineered recombinant protein products of the avian paramyxovirus type-1 nucleocapsid and phosphoprotein genes for serological diagnosis

Fig. 2

Assembled Western blot assays of monobiotinylated recombinant proteins of NDV LaSota. Panels a and b were stained using a monoclonal antibody specific for biotin. For panel c a chicken serum (S880/Hu295) was used that was obtained at day 28 after immunization with live-attenuated NDV LaSota vaccine. Panels a and c were run under denaturing conditions while panel b was obtained after protein separation in native PAGE. M – marker lane, NP – full length nucleocapsid protein; NPct -C-terminal fragment of nucleocapsid protein; Pu – unique C-terminal part of phosphoprotein; Vu – unique C-terminal part of V protein; C4BP – heptamerization domain of human C4 binding protein; N – Rosettagami E. coli lysate. Molecular weights of monomeric recombinant proteins (panels a, c) and multimeric conglomerates (panel b) are presented to the left of the marker lane

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